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LC Sciences microrna microarray data analysis
Microrna Microarray Data Analysis, supplied by LC Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microrna+microarray/mirna+microarray/us12311034-426-0-5
Average 90 stars, based on 1 article reviews
microrna microarray data analysis - by Bioz Stars, 2026-09
90/100 stars

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Microarray:

Article Title: Monolayer culture alters EGFR inhibitor response through abrogation of microRNA-mediated feedback regulation
Article Snippet: .. The microRNA microarray was performed by LC Sciences (Houston, TX; RRID:SCR_000140) using μParafloTM chip technology with probes for all miRNA listed in Sanger miRBase Release 21 (mirBase.org, RRID:SCR_003152)). .. miR-146a-5p was quantified from RNA via reverse transcriptase qPCR, using miRCURY LNA miRNA SYBR Green RT-PCR Kit (Qiagen #339345) with miR-146a-5p-specific primers (Qiagen #YP00204688) as per the manufacturer’s instructions.

Article Title: MicroRNAs and immunity in periodontal health and disease
Article Snippet: .. To determine whether miRNAs are involved in the regulation of the immune response during periodontitis progression, we performed miRNA expression profiling analysis using our periodontitis animal model. For our analysis, gingival crevices from healthy and diseased animals were dissected, and miRNA expression profiles were compared using a microRNA microarray (LC Sciences, Houston, TX, USA). ..

Article Title: TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation
Article Snippet: .. © 2015 American Association for Cancer Research. cancerres.aacrjournals.org Downloaded from 9 MicroRNA microarray and next generation RNA sequencing 10 μg total RNA was isolated using the miRNeasy Mini Kit (Qiagen) and was submitted in triplicate to LC Sciences for microRNA microarray services and data analysis using miRBase v15.0. ..

Article Title: Poster Sessions: Basic
Article Snippet: .. We assessed the difference of miRNAs expression between graft and control groups by using microRNA microarray (miRbase19.0, μParafl o® Microfl uidic Biochip from LC Sciences) and Quantitative PCR analysis, followed by predicting miRNA putative targets (TargetScan) and pathways using pathway-mapping algorithms (Gene Ontology and KEGG). ..

Article Title: Over-expression of miR-3584-5p Represses Nav1.8 Channel Aggravating Neuropathic Pain caused by Chronic Constriction Injury.
Article Snippet: Nav1.8, a tetrodotoxin-resistant voltage-gated sodium channels (VGSCs) subtype encoded by SCN10A, which plays an important role in the production and transmission of peripheral neuropathic pain signals.. Studies have shown that VGSCs may be key targets of MicroRNAs (miRNAs) in the regulation of neuropathic pain.. In our study, bioinformatics analysis showed that the targeting relationship between miR-3584-5p and Nav1.8 was the most closely.

Article Title: MSC Transplantation Improves Osteopenia via Epigenetic Regulation of Notch Signaling in Lupus
Article Snippet: For microRNA microarray analysis, total RNA from cells at passage one was extracted using a miRNeasy Mini Kit (Qiagen) according to the manufacturer’s instructions. .. MicroRNA microarray and data processing were performed by LC Sciences (Houston, TX, USA). ..

Article Title: miR-598 inhibits metastasis in colorectal cancer by suppressing JAG1/Notch2 pathway stimulating EMT.
Article Snippet: MicroRNAs (miRNAs) are a class of endogenous, evolutionarily conserved small non-coding RNA molecules that mediate the posttranscriptional process of target gene, leading to translational repression or degradation of target mRNAs.. A series of studies have indicated that miRNAs play an important role in tumor initiation, development and progression.. In this study, we found that down regulation of miR-598 was a frequent event in CRC tissues compared to the paracarcinoma tissues.

Article Title: miR-3156-3p is downregulated in HPV-positive cervical cancer and performs as a tumor-suppressive miRNA
Article Snippet: .. The miRNA expression profiles were identified in HT-3E6/E7, HT-3 V and HT-3 cell lines using an LC Sciences microRNA microarray(Hangzhou, China) containing 2578 human mature microRNAs based on Sanger miRBase Release 20.0. ..

Expressing:

Article Title: MicroRNAs and immunity in periodontal health and disease
Article Snippet: .. To determine whether miRNAs are involved in the regulation of the immune response during periodontitis progression, we performed miRNA expression profiling analysis using our periodontitis animal model. For our analysis, gingival crevices from healthy and diseased animals were dissected, and miRNA expression profiles were compared using a microRNA microarray (LC Sciences, Houston, TX, USA). ..

Article Title: Poster Sessions: Basic
Article Snippet: .. We assessed the difference of miRNAs expression between graft and control groups by using microRNA microarray (miRbase19.0, μParafl o® Microfl uidic Biochip from LC Sciences) and Quantitative PCR analysis, followed by predicting miRNA putative targets (TargetScan) and pathways using pathway-mapping algorithms (Gene Ontology and KEGG). ..

Article Title: miR-3156-3p is downregulated in HPV-positive cervical cancer and performs as a tumor-suppressive miRNA
Article Snippet: .. The miRNA expression profiles were identified in HT-3E6/E7, HT-3 V and HT-3 cell lines using an LC Sciences microRNA microarray(Hangzhou, China) containing 2578 human mature microRNAs based on Sanger miRBase Release 20.0. ..

Animal Model:

Article Title: MicroRNAs and immunity in periodontal health and disease
Article Snippet: .. To determine whether miRNAs are involved in the regulation of the immune response during periodontitis progression, we performed miRNA expression profiling analysis using our periodontitis animal model. For our analysis, gingival crevices from healthy and diseased animals were dissected, and miRNA expression profiles were compared using a microRNA microarray (LC Sciences, Houston, TX, USA). ..

RNA Sequencing:

Article Title: TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation
Article Snippet: .. © 2015 American Association for Cancer Research. cancerres.aacrjournals.org Downloaded from 9 MicroRNA microarray and next generation RNA sequencing 10 μg total RNA was isolated using the miRNeasy Mini Kit (Qiagen) and was submitted in triplicate to LC Sciences for microRNA microarray services and data analysis using miRBase v15.0. ..

Isolation:

Article Title: TWIST1-Induced miR-424 Reversibly Drives Mesenchymal Programming while Inhibiting Tumor Initiation
Article Snippet: .. © 2015 American Association for Cancer Research. cancerres.aacrjournals.org Downloaded from 9 MicroRNA microarray and next generation RNA sequencing 10 μg total RNA was isolated using the miRNeasy Mini Kit (Qiagen) and was submitted in triplicate to LC Sciences for microRNA microarray services and data analysis using miRBase v15.0. ..

Control:

Article Title: Poster Sessions: Basic
Article Snippet: .. We assessed the difference of miRNAs expression between graft and control groups by using microRNA microarray (miRbase19.0, μParafl o® Microfl uidic Biochip from LC Sciences) and Quantitative PCR analysis, followed by predicting miRNA putative targets (TargetScan) and pathways using pathway-mapping algorithms (Gene Ontology and KEGG). ..

Real-time Polymerase Chain Reaction:

Article Title: Poster Sessions: Basic
Article Snippet: .. We assessed the difference of miRNAs expression between graft and control groups by using microRNA microarray (miRbase19.0, μParafl o® Microfl uidic Biochip from LC Sciences) and Quantitative PCR analysis, followed by predicting miRNA putative targets (TargetScan) and pathways using pathway-mapping algorithms (Gene Ontology and KEGG). ..



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a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The <t>microRNA</t> profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.
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Image Search Results


a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

Journal: Cell Death & Disease

Article Title: A novel TGFbeta/TGILR axis mediates crosstalk between cancer-associated fibroblasts and tumor cells to drive gastric cancer progression

doi: 10.1038/s41419-024-06744-0

Figure Lengend Snippet: a , b The overexpression efficiency of TARBP2 was examined by western blotting assay. c , d TARBP2 overexpression significantly inhibits the proliferation and invasion of GC cell lines. e The microRNA profiling array was performed to identify the miRNAs regulated by TARBP2 overexpression in the AGS cell line. f The expression correlation between TARBP2 and per microRNA in TCGA stomach cancer cohort were shown in the volcano plot. TARBP2 was highly co-expressed with miR-1306 and miR-33a in GC. g The overall survival analysis of miR-1306 and miR-33a in the TCGA_STAD cohort. h , i TARBP2 overexpression significantly upregulated the expression levels of miR-1306 and miR-33a in GC cell lines. j The rescue assay confirmed that inhibition of miR-1306 and miR-33a can partially restore the inhibitory effect of TARBP2 overexpression on GC cell proliferation. The inhibitor-poor means the mixture of miR-1306 inhibitor and miR-33a inhibitor.

Article Snippet: Thus, a high-throughput Agilent microRNA profile microarray was performed to identify the differentially expressed microRNAs after TARBP2 overexpression.

Techniques: Over Expression, Western Blot, Expressing, Rescue Assay, Inhibition

(A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

Journal: PLOS ONE

Article Title: MicroRNA-26a-5p is a reliable biomarker in the adjuvant setting for pancreatic ductal adenocarcinoma

doi: 10.1371/journal.pone.0310328

Figure Lengend Snippet: (A) Flowchart of candidate miRNA selection. Microarray results were compared between patients with and without subclinical tumors or recurrence under or after adjuvant chemotherapy, and candidate miRNAs were isolated. (B) Volcano plots for candidate miRNAs in patients with recurrence during AC. ( C)(D) GSEA comparing Panc1-GR and Panc1-Pt cells. GSEA-extracted representative gene sets enriched in these cells are shown. ( E) List showing the ranking of candidate miRNAs from the above 5 miRNAs according to the percentage of genes related to the integrin-mediated cell adhesion pathway retrieved by TargetScan.

Article Snippet: To investigate candidate miRNAs, microarray analyses of miRNA expression in 18 blood samples ( ) was performed using the Toray microRNA microarray system (Toray Industries, Tokyo, Japan).

Techniques: Selection, Microarray, Adjuvant, Isolation